Profile Pemenang Juara 3 Best Research 2014

  • Nama : Dr. Drh. NLP Indi Dharmayanti, M.Si
  • TTL : Banyuwangi, 10 Mei 1972
  • Jabatan : Ketua Kelompok Peneliti Virologi
  • Institusi : Balai Besar Penelitian Veteriner, Kementerian Pertanian
  • Alamat : JL RE Martadinata no 30, Bogor 16114, Jawa Barat
  • Email : nlpdharmayanti@gmail.com

Pendidikan

No.

Tingkat

Jurusan

Nama & Tempat
Perguruan Tinggi

Tahun Lulus

1

S1-Profesi

Kedokteran Hewan

Universitas Airlangga, Surabaya

1997

2

S2

Bioteknologi

Universitas Gadjah Mada, Jogjakarta

2003

3

S3

Biomedis

Universitas Indonesia

2009

 

Pengalaman Profesional

No.

Tahun

Posisi

Bidang Pekerjaan

Perusahaan/Institusi

 

2013-sekarang

Ketua Kelompok Peneliti Virologi/Kepala Laboratorium Virologi

Virologi

Balai Besar Penelitian Veteriner

 

2013-sekarang

Anggota Komisi Keamanan Hayati, TTKH, Kementerian Lingkungan Hidup

Narasumber bidang Rekayasa genetika Jasad Renik

Kementerian Lingkungan Hidup

 

2012-sekarang

Deputy Biosafety officer, Deputy Manager BSL-3 Moduler

Biosafety, Biosecurity

Balai Besar Penelitian Veteriner

 

2014-sekarang

Anggota Penilai Pendaftaran Obat Hewan (PPOH)

Narasumber

Direktorat Jendral Peternakan dan Kesehatan Hewan

 

2013

Anggota Komisi Kesehatan Hewan

Narasumber

Direktorat Jendral Peternakan dan Kesehatan Hewan

 

2013-sekarang

Anggota Komisi Kesejahteraan Hewan Coba

Narasumber

Balai Besar Penelitian Veteriner

 

2014

Aggota Influenza Monitoring System

Narasumber

Direktorat Jendral Peternakan dan Kesehatan Hewan

 

2014

Tim Integrator Badan LitBangTan

Integrator

Badan LitBang Pertanian

 

Bidang Ilmu Penelitian

Diagnostik/Metode pengobatan

Judul Penelitian

Genetic characterization of clade 2.3.2.1 avian influenza A (H5N1) viruses, Indonesia, 2012

Tahun Penelitian

2012-2013

Dampak/Aplikasi Hasil Penelitian

Hasil penelitian ini menginformasikan adanya introduksi virus avian influenza H5N1 baru di Indonesia, ditengah situasi endemis virus avian influenza H5N1 lama. Hal ini  kemungkinan akan menimbulkan dampak terhadap kesehatan hewan dan manusia. Karakter virus H5N1 baru ini yaitu virus clade 2.3.2.1 ini  dikarenakan : 

  1. Virus H5N1 clade 2.3.2.1 asal Indonesia berbeda 8-10 asam amino dengan virus kandidat vaksin H5N1 clade 2.3.2.1. untuk manusia yang direkomendasikan oleh WHO (A/Hubei/1/2010).
  2. Virus H5N1 clade 2.3.2.1 yang direaksikan dengan antiserum referens virus H5N1asal manusia calde 1, clade 2.2.1, 2.3.4, 2.1.3.2, 2.3.2.1 (asal Mongolia, Hong Kong dan VietNam) menunjukkan bahwa secara antigenik virus clade 2.3.2.1 asal Indonesia mempunyai kedekatan antigenik dengan  virus kelompok A/HongKong/6841/2010 -like
  3. Terdapat virus H5N1 clade 2.3.2.1 menunjukkan penurunan kepekaan terhadap obat antifluburung (osetamivir)

Jika terjadi wabah/infeksi manusia di Indonesia karena virus ini kemungkinan kandidat vaksin H5N1 yang direkomendasikan WHO tidak akan sesuai dengan virus clade 2.3.2.1 yang bersirkulasi di Indonesia.   Deteksi clade novel A (H5N1) virus di Indonesia menandai titik balik potensial dalam epidemiologi molekuler virus ini. Indonesia memiliki jumlah tertinggi dari manusia (H5N1) infeksi A karena wabah yang sedang berlangsung pada unggas (14,15). Apakah virus baru ini akan menjadi berurat berakar, seperti yang dilakukan clade 2.1.3 virus selama dekade terakhir, masih harus dilihat, seperti halnya efek pada kejadian infeksi pada manusia. Potensi cocirculation dari subtipe yang berbeda 2 clade ini harus dilakukan kaji ulang terhadap metode diagnostik dan strategi vaksinasi untuk memaksimalkan efektivitas intervensi pengendalian penyakit. Kurangnya keterkaitan antigenik antara clade 2.3.2.1 dan 2.1.3.2 virus harus dipertimbangkan ketika mengevaluasi A (H5N1) serologi reagen diagnostik yang digunakan di Indonesia. Perubahan ini juga mungkin memiliki implikasi dalam memilih prepandemic virus vaksin kandidat untuk wilayah tersebut.


 Abstrak Penelitian

We describe the genetic characteristics of viruses isolated from A(H5N1) infection outbreaks in duck farma in  3 provinces on Java Island, where a previously unrecognized clade was detected. Samples showing suspected A(H5N1) infection were propagated in a Biosafety Level-3 laboratory at IRCVS in compliance with biosafety regulations.  The virus isolates from duck farms and  LBMs were chosen for full-length HA gene were selected for genome sequencing. Phylogenetic trees were generated by using MEGA4. Phylogenetic analysis revealed that A(H5N1) isolates from samples collected from duck farm outbreaks and an LBM were not related to isolates in long-established Indonesian clade 2.1; rather, the HA genes closely resembledthose of clade 2.3.2.1 viruses recently found in Vietnam, China, and Hong Kong.  Full-length HA genes showed 97%–98%-nt identity with recent viruses from Vietnam and clustered in a larger group containing viruses from many Asian regions during 2009–2012. To test the antigenic relationship of the clade 2.3.2.1 virus to the endemic clade 2.1.3.2 virus, we conducted a hemagglutination-inhibition test with ferret antiserum raised against viruses from these and other H5N1 clades. As the HA1 protein sequence differences suggest, clade 2.1.3.2 antiserum did not inhibit hemagglutination by a representative clade 2.3.2.1 virus from Indonesia, A/environment/East Java/LBM-LM13/2012. In contrast, this virus cross-reacted with antiserum to clade 2.3.2.1 viruses from other countries at heterologous titers generally within 2-fold of or equivalent to the homologous virus titer. The Indonesian clade 2.3.2.1 virus was most closely related antigenically to viruses that clustered genetically into the A/Hong Kong/6841/2010-like group of clade 2.3.2.1. In contrast, the HA1 of the new viruses collected in Indonesia differed by 8–10 aa from A/Hubei/1/2010, the most closely related clade 2.3.2.1 A(H5N1) candidate vaccine virus recommended by the World Health Organization. Although 1 sample had an Ile203Val substitution in the NA, which has been associated with reduced susceptibility to oseltamivir, no other markers of resistance in the NA or M2 were identified. Detection of a novel clade of A(H5N1) virus in Indonesia marks a potential turning point in the molecular epidemiology of this virus. Indonesia has the highest number of human A(H5N1) infections because of ongoing outbreaks in poultry (14,15). Whether this new virus will become entrenched, as did clade 2.1.3 viruses over the past decade, remains to be seen, as do its effects on the incidence of human infection. Potential cocirculation of subtypes of 2 different clades warrants review of diagnostic methods and vaccination strategy to maximize effectiveness
of disease control interventions. The lack of antigenic relatedness between the clade 2.3.2.1 and 2.1.3.2 viruses must be considered when evaluating A(H5N1) serologic diagnostic reagents used in Indonesia. This change also may have implications in selecting prepandemic candidate vaccine virus for the region.


 

Nama Peneliti

  1. Ni Luh Putu Indi Dharmayanti
  2. Risza Hartawan
  3. Pudjiatmoko
  4. Hendra Wibawa
  5. Hardiman
  6. Amanda Balish
  7. Ruben Donis
  8. C. Todd Davis
  9. Gina Samaan

Penghargaan yang pernah Didapatkan

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Publikasi yang pernah Diterbitkan

  1. Ni Luh Putu Indi Dharmayanti, Fera Ibrahim, Darminto, Amin Soebandrio. 2011. Influenza H5N1 Virus of Birds Surrounding H5N1 Human Cases Have Specific characteristics on the Matrix Protein. HAYATI Journal of Biosciences.18.2 : 82-90
  2. Ni Luh Putu Indi Dharmayanti, Gina Samaan, Fera Ibrahim, Risa Indriani, Darminto, Amin Soebandrio. 2011. The Genetic Drift of Indonesian Avian Influenza A H5n1 Viruses During 2003-2008. Microbiology Indonesia. 5.2 : 68-80
  3. Ni Luh Putu Indi Dharmayanti, Fera Ibrahim, Amin Soebandrio. 2010. Amantadine Resistant of Indonesian H5N1 subtype Influenza viruses during 2003-2008. Microbiology Indonesia. 4. 1 : 11-16
  4. Ni Luh Putu Indi Dharmayanti, Atik Ratnawati, Dyah Ayu Hewajuli, Risa Indriani 2014. Genetic characterization of H5N1 avian influenza viruses isolated from pet bird and chickens from live bird market in Bali and Bekasi (Indonesia), 2011. AJMR. 8.3 :  244-251
  5. Ni Luh Putu Indi Dharmayanti, Risza Hartawan, Pudjiatmoko, Hendra Wibawa, Hardiman, Amanda Balish, Ruben Donis, C. Todd Davis, and Gina Samaan. 2014. Genetic Characterization of Clade 2.3.2.1 Avian Influenza A(H5N1) Viruses, Indonesia, 2012. Emerging Infectious Diseases. 20. 4 :  671-674